Aims and Scope
Recent Advances in the Ecoepidemiology, Virulence and Diagnosis of Cryptococcus neoformans and Cryptococcus gattii Species ComplexesKebabonye Kenosi, Jongman Mosimanegape, Loeto Daniel and Kasvosve Ishmael
The Open Microbiology Journal, 2023; 17: e187428582303270.
Electronic Publication Date: May 17, 2023
Evaluation of the Impact of some Plant Extracts against Streptococcus Spp. Isolated from Dental Decay InfectionAnas Abdullah Hamad, Maryam S. Alhumaidi and Azadeh Manayi
The Open Microbiology Journal, 2023; 17: e187428582303060.
Electronic Publication Date: May 09, 2023
Network of Interaction among Enterobacter Species and Klebsiella Pneumonia Clinical Isolates and the Antibiotic Resistance Pattern at Cape CoastKwame Kumi Asare, Jennifer Mbata, Samuel Amoah, Peter Bilatam Mayeem, Felicity Bentsi-Enchill and Yeboah Kwaku Opoku
The Open Microbiology Journal, 2023; 17: e187428582212160.
Electronic Publication Date: February 06, 2023
Identification of Novel Mobile Genetic Elements Associated with Resistance to Macrolide and Lincosamide in Streptococcus dysgalactiae subsp. equisimilisAlexandra Kireeva and Alexander Dmitriev
The Open Microbiology Journal, 2023; 17: e187428582212300.
Electronic Publication Date: January 31, 2023
Comparison of Dot ELISA Using GroEL Recombinant Protein as an Antigen and an Indirect Hemagglutination Assay for Serodiagnosis of MelioidosisKanyanan Kritsiriwuthinan, Sumet Wajanarogana, Kantima Choosang, Thitima Pimklang
Melioidosis is a disease caused by the Burkholderia pseudomallei bacterium. The mortality rate of infected patients is quite high because the symptoms are similar to those of various diseases, making it difficult to diagnose clinically and preventing the immediate treatment with effective antibiotics that is required for the management of acute infections. To provide appropriate treatment, accurate and rapid diagnosis is required.
The aims of this study were to develop Dot ELISA using purified GroEL B. pseudomallei recombinant protein as an antigen and to compare the newly developed assay with an indirect hemagglutination assay (IHA) for the diagnosis of melioidosis.
The GroEL recombinant protein was purified by immobilized metal affinity chromatography before being used as an antigen. The optimal conditions of the Dot ELISA were determined and used for subsequent experiments. A total of 291 serum samples were evaluated by the established Dot ELISA and IHA, using the bacterial culture method as the gold standard of melioidosis diagnosis.
The results from Dot ELISA and IHA revealed sensitivity, specificity, and accuracy of 85.7% (Dot ELISA)/64.3% (IHA), 94.4%/85.5%, and 93.1%/82.5%, respectively.
These results indicate that the Dot ELISA developed is an efficient, simple, rapid and cost-effective technique for the early diagnosis of melioidosis and can be used in a local laboratory without specialized equipment.
March 22, 2021
- April 23, 2020
Prevalence of Human Papillomavirus Associated with Head and Neck Squamous Cell Carcinoma in Jordanian PatientsDecember 15, 2020
A Review on Biochemical and Immunological Biomarkers used for Laboratory Diagnosis of SARS-CoV-2 (COVID -19)May 31, 2019
An Epidemiological Review on Emerging and Re-Emerging Parasitic Infectious Diseases in MalaysiaFebruary 28, 2019
Distribution of Class 1 and 2 Integrons Among Salmonella Enterica Serovars Isolated from Iranian PatientsSeptember 30, 2019
High Potential Risk of Zika Virus Infection Outbreak in Dengue Suspected Cases in NepalAugust 31, 2018
Composition of Intestinal Microbiota in Two Lines of Rainbow Trout (Oncorhynchus Mykiss) Divergently Selected for Muscle Fat Content