Polymorphic Amplified Typing Sequences (PATS) Strain Typing System Accurately Discriminates a Set of Temporally and Spatially Disparate Escherichia coli O157 Isolates Associated with Human Infection



Indira T. Kudva1, *, Sandra Smole2, Robert W. Griffin3, Jeonifer Garren3, Nimisha Kalia2, Megan Murray3, 4, Manohar John3, 5, , Ralph Timperi2, ††, Stephen B. Calderwood3, 5, 6, *
1 Food Safety and Enteric Pathogens Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, IA. 50010
2 William A. Hinton State Laboratory Institute, Massachusetts Department of Public Health, Jamaica Plain, MA 02130
3 Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114
4 Department of Epidemiology, Harvard School of Public Health, Boston, MA 02115
5 Department of Medicine, Harvard Medical School, Boston, MA 02115
6 Department of Microbiology and Immunobiology Harvard Medical School Boston, MA 02115 †Pathovacs Inc., Ames, IA Association of Public Health Laboratories, Silver Spring, MA
Pathovacs Inc., Ames, IA
†† Association of Public Health Laboratories, Silver Spring, MA


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© Kudva et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to these authors at the Division of Infectious Diseases, Massachusetts General Hospital, Boston, MA 02114; Tel: 617-726-3811; Fax: 617-726-7416; E-mail: scalderwood@partners.org; Food Safety and Enteric Pathogens Research Unit, National Animal Disease Center/ARS/USDA, 1 North, Bldg. 20, 1121, Ames, IA. 50010; Tel: 515-337-7376; Fax: 515-337-7438; E-mail: Indira.Kudva@ars.usda.gov


Abstract

Polymorphic Amplified Typing Sequences (PATS) is a PCR-based Escherichia coli O157 (O157) strain typing system. Here, we show that PATS compares excellently with Pulsed-Field Gel Electrophoresis (PFGE) in that both methods cluster geographically diverse O157 isolates similarly. Comparative analysis of the results obtained in this simulated “blind” study attests to the discriminating power and applicability of PATS to epidemiological/nosocomial situations.

Keywords: Bacteria, O157, Strain-typing, PCR, PATS, PFGE.