RESEARCH ARTICLE


Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry



Reiner Schaumann1, , Nicolas Knoop1, , *, Gelimer H Genzel1, Kevin Losensky1, Christiane Rosenkranz1, Catalina S Stîngu1, Wolfgang Schellenberger2, Arne C Rodloff1, Klaus Eschrich2
1 Institute for Medical Microbiology and Epidemiology of Infectious Diseases, University of Leipzig, Leipzig, Germany
2 Institute of Biochemistry, School of Medicine, University of Leipzig, Leipzig, Germany


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© Schaumann et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/-licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to these authors at the Department for Medical Microbiology and Epidemiology of Infectious Diseases, University Hospital Leipzig, University of Leipzig, Liebigstr. 20, D-04103 Leipzig, Germany; Tel: +49 341 97 17 947; Fax: +49 341 97 17 539; E-mail: nicolas.knoop@medizin.uni-leipzig.de
Both authors contributed equally to this work


Abstract

Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry

Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has proven to be an effective identification tool in medical microbiology. Discrimination to subspecies or serovar level has been found to be challenging using commercially available identification software. By forming our own reference database and using alternative analysis methods, we could reliably identify all implemented Enterobacteriaceae and non-fermenting gram negative bacilli by MALDI-TOF MS and even succeeded to distinguish Shigella sonnei from Escherichia coli (E. coli) and Salmonella enterica spp. enterica serovar Enteritidis from Salmonella enterica spp. enterica serovar Typhimurium. Furthermore, the method showed the ability to separate Enterohemorrhagic E. coli (EHEC) and Enteropathogenic E. coli (EPEC) from non-enteropathogenic E. coli.

Keywords:: E. coli, EHEC, Enterobacteriaceae, EPEC, MALDI-TOF MS, Salmonella spp, Shigella sonnei.