Characterization of Vibrio cholerae O139 of an Aquatic Isolate in Northern Vietnam
Dong Tu Nguyen 1, 2, Tuan Cuong Ngo 1, Huy Hoang Tran 1, Thanh Huong Le 1, Hoai Thu Nguyen 1, Binh Minh Nguyen1, Nhu Duong Tran 3, Tetsu Yamashiro 4, Masahiko Ehara 5, *
Identifiers and Pagination:Year: 2012
First Page: 14
Last Page: 21
Publisher Id: TOMICROJ-6-14
Article History:Received Date: 12/10/2011
Revision Received Date: 17/12/2011
Acceptance Date: 21/12/2011
Electronic publication date: 10/2/2012
Collection year: 2012
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
During the cholera survey in Namdinh province (northern Vietnam) in July, 2010, one strain of Vibrio cholerae O139 was isolated from 7 environmental water samples positive for ctxA, toxR,VCO139 genes and named as V. cholerae O139, ND1 strain. This strain was lysogenic harbouring a genome similar to the filamentous phage fs1. The replicative form DNA of this phage (named as ND1-fs1, 6856 bp) was sequenced and compared with the other filamentous phages. The filamentous phage ND1-fs1 integrates into the region between ctxB and rtxA genes. The genetic organization of the CTXϕ of V. cholerae O139, strain ND1 was determined and the schematic representation of the genetic organization was shown together with the ND1-fs1 prophage.