RESEARCH ARTICLE


The Complete Genome Sequence of Bacillus safensis BRM1 Isolated from Brazilian Mangrove Sediment: A Potential Source of Biomass Converting Enzymes



Marcelo Scarduelli1, *, Dieval Guizelini2, Rodrigo Luis Alves Cardos3, Denny Marcel Ceccon1, Lucélia Donatti4, Valter Antônio de Baura1, Fábio de Oliveira Pedrosa1, Luciano Fernandes Huergo1, Emanuel Maltempi de Souza1
1 Programa de Pós-Graduação em Ciências – Bioquímica, Departamento de Bioquímica e Biologia Molecular, UFPR, Curitiba, PR, Brazil
2 Setor de Educação Profissional e Tecnológica da UFPR, Curitiba, PR, Brazil
3 Departamento de Bioquimicae Biologia Moleculars, Federal University of Paraná, Brazil
4 Departamento de Biologia Celular, UFPR, Curitiba, PR, Brazil


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Creative Commons License
© 2022 Scarduelli et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Programa de Pós-Graduação em Ciências – Bioquímica, Departamento de Bioquímica e Biologia Molecular, UFPR, Curitiba, PR, Brazil; E-mail: marcelomogoda@gmail.com


Abstract

Background:

Bacillus safensis BRM1 was isolated from Brazilian mangrove sediment and selected for its ability to grow in xylan as the sole carbon source. To identify genes encoding biomass conversion enzymes, the genome of this bacterium was sequenced.

Methods:

Genome wide analysis revealed 99% nucleotide identity to the Bacillus safensis genome. The isolated strain was named B. safensis BRM1, and its genome consists of a circular chromosome of 3.74 Mb with a GC content of 41.8%. Genes encoding a plethora of hydrolytic enzymes are present in the BRM1 genome but absent from the other B. safensis genomes.

Results:

A total of 23 genes encoding putative cellulases or hemicellulases were identified.

Conclusion:

These data support that B. safensis BRM1 is an interesting candidate for the prospection of enzymes that can be applied in the conversion of cellulosic biomass to biofuel.

Keywords: Bacillus safensis, Xylanases, Mangrove, Genes encoding, Enzymes, Genome.