RESEARCH ARTICLE


Listeriolysin O, but not Murine E-cadherin, is Involved in Invasion of Listeria monocytogenes into Murine Liver Parenchymal Cells



Yu-ju Kanayama, Masakazu Kaneko, Yoshiko Emoto, Masashi Emoto*
Laboratory of Immunology, Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan


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© Kanayama et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Laboratory of Immunology, Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, 3-39-22 Showa-machi, Maebashi, Gunma 371-8511, Japan; Tel: +81-27-220-8935; Fax: +81-27-220-8935; E-mail: masashi.emoto@gmail.com


Abstract

Human E-cadherin and listeriolysin O (LLO) are involved in invasion of Listeria monocytogenes into human liver parenchymal cells (LPC). Yet, it remains to be determined whether murine E-cadherin and LLO participate in invasion of L. monocytogenes into murine LPC. In the present study, involvement of murine E-cadherin and LLO in invasion of L. monocytogenes into murine LPC was investigated. Murine E-cadherin was expressed on murine LPC, but the expression became undetectable by insertion of transgene of Simian virus 40 large T antigen. Although invasion of L. monocytogenes into murine LPC was found regardless of murine E-cadherin expression, infection rate of L. monocytogenes being unable to secrete LLO was lower than that of L. monocytogenes being capable of secreting LLO. Our RESULTS verify that invasion of L. monocytogenes into murine LPC occurs independently of murine E-cadherin and indicate that LLO participates in invasion of L. monocytogenes into murine LPC.

Keywords: E-cadherin, Hepatocyte, Internalin, Invasion, Listeria monocytogenes, Listeriolysin O.