RESEARCH ARTICLE


Pre-induced Lac Operon Effect on Non Specific Sugars: Pre-culture Effect is Dependent on Strength of Induction, Exponential Phase and Substrate Concentration



Pushkar Malakar*
Department of Bioscience & Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai-400076, Maharashtra, India


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© Pushkar Malakar; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Biosciences and Bioengineering, Indian Institute of Technology Bombay, Powai, Mumbai-400076, Maharashtra, India; Tel: +91-22-2576 4215; Fax: +91-22-2572 6895; E-mails: pushkarbt@iitb.ac.in;pushkarbt@gmail.com


Abstract

The source and history of the cell plays an important role in influencing the phenotypic properties of the organism in a particular environmental condition. Pre-induced lac operon provides benefit on lactose environment. During metabolism lactose is broken down into glucose and galactose. The fate of cells with pre-induced lac operon on glucose and galactose milieu is not known. The influence of nutritional status of the medium, level of pre-induction and growth phase on pre-culture effect is not investigated. Effect of pre-induced lac operon on non specific sugars along with the factors that influence this effect was enumerated in the present study. Results of this present study indicate that pre-induced lac operon provide benefit in terms of growth on galactose milieu. This study also suggests that Pre induced lac operon effect depends on the (i) strength of induction in the pre-culture, (ii) nutritional content of the environment and (iii) exponential growth phase of the organism. The above study will help in the better characterization of the pre culture effect. It will also help in the better understanding of the relation between gene expression and growth physiology.

Keywords: Entry into Early Exponential Phase, Galactose, IPTG and Strength of Induction, Late Exponential Phase, Pre-induced lac operon.