Evaluation of Immunoprotective Activity of Six Leptospiral Proteins in the Hamster Model of Leptospirosis
Marina V Atzingen1, Mônica L Vieira1, 3, Rosane Oliveira1, 3, Renan F Domingos1, 3, Renata S Mendes1, 3, Aline T Barros1, Amane P Gonçales2, Zenaide M de Morais2, Silvio A Vasconcellos2, Ana LTO Nascimento1, 3, *
Identifiers and Pagination:Year: 2012
First Page: 79
Last Page: 87
Publisher ID: TOMICROJ-6-79
Article History:Received Date: 01/6/2012
Revision Received Date: 23/7/2012
Acceptance Date: 18/7/2012
Electronic publication date: 19/10/2012
Collection year: 2012
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Leptospirosis is a worldwide zoonosis caused by pathogenic Leptospira. The whole-genome sequence of L. interrogans serovar Copenhageni together with bioinformatics tools represent a great opportunity to search for novel antigen candidates that could be used as subunit vaccine against leptospirosis. We focused on six genes encoding for conserved hypothetical proteins predicted to be exported to the outer membrane. The genes were amplified by PCR from Leptospira interrogans genomic DNA and were cloned and expressed in Escherichia coli. The recombinant proteins tagged with N-terminal hexahistidine were purified by metal-charged chromatography. The immunization of hamsters followed by challenge with lethal dose of virulent strain of Leptospira showed that the recombinant proteins Lsa21, Lsa66 and rLIC11030 elicited partial protection to animals. These proteins could be used combined or in a mixture with novel adjuvants in order to improve their effectiveness.