All published articles of this journal are available on ScienceDirect.
Influence of Environmental Factors on Biofilm Formation by Heat-resistant Spore-forming Bacillus cereus TGS11.1
iD
Bich Hanh Bui Thi1
iD
Thanh Huy Tran Huu1
iD
The Vinh Bui1
iD
Thi-Kim-Dung Hoang2
iD
Khoa Anh Ton1
iD
Authors Info & Affiliations
Abstract
Introduction/Objective
This study evaluates the synergistic influence of environmental factors on the biofilm formation capacity of the heat-resistant, spore-forming pathogen Bacillus cereus TGS11.1 within milk-processing contexts.
Methods
Response Surface Methodology with a Central Composite Design (RSM-CCD) was implemented to quantify the multifactorial effects of temperature (30–70°C), pH (4–8), lactose concentration (2–6%), and incubation time (12–60 h) on biofilm development using optical density measurements and phenotypic motility assays.
Results
Temperature emerged as the dominant determinant of biofilm formation, with peak productivity and OD570 values occurring at 40°C, correlating with maximum swarming motility and the establishment of a dense three-dimensional (3D) architectural network.
Discussion
The observed sensitivity of B. cereus to thermal gradients and nutrient availability underscores the need for multifactorial intervention strategies to disrupt the structural resilience of the biofilm matrix during dairy production.
Conclusion
Mitigating recontamination risks in dairy workflows necessitates the strict avoidance of thermal niches near 40°C to inhibit the optimal physiological conditions for robust biofilm formation by B. cereus TGS11.1.