RESEARCH ARTICLE


Clinico-Pathological Study of Adenovirus Associated with Respiratory Infections in Children



Maysaa El Sayed Zaki1, *
iD
, Abd R. Eid2
iD
, Osama A. Faried3
iD

1 Department of Clinical Pathology Faculty of Medicine-Mansoura University Mansoura, Egypt
2 Department of Pediatric- Genetics Unit Faculty of Medicine-Mansoura University Mansoura, Egypt
3 Medical Microbiology and Immunology-Medical Microbiology and Immunology-Beni Sued Faculty of Medicine-Beni Suef University, Mansoura, Egypt


Article Metrics

CrossRef Citations:
0
Total Statistics:

Full-Text HTML Views: 279
Abstract HTML Views: 65
PDF Downloads: 0
Total Views/Downloads: 344
Unique Statistics:

Full-Text HTML Views: 198
Abstract HTML Views: 54
PDF Downloads: 0
Total Views/Downloads: 252



Creative Commons License
© 2020 Zaki et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Correspondence: Address correspondence to this author at Department of Clinical Pathology Faculty of Medicine-Mansoura University Mansoura, Mansoura, Egypt;
E-mail: may_s65@hotmail.com


Abstract

Background:

Adenovirus is associated with respiratory tract infections in children worldwide. However, there is insufficient data about adenovirus infections in Egyptian children and the genotypes present in this infection.

Objective:

The aim of the present study was to investigate the prevalence of adenovirus and its genotypes in respiratory tract infection in children by real-time Polymerase Chain Reaction (PCR).

Methods:

The study was a cross-sectional study that included 100 children complaining of respiratory tract infections signs and symptoms. Laboratory investigation for adenovirus included real-time polymerase chain reaction and genotypes detection by Multiplex Polymerase Chain Reaction (PCR).

Results:

Adenovirus was detected by PCR for fiber gene in 11% with genotype 3 in 6 samples (54.5%) and genotype 7 in 5 samples (45.5%) positive for adenovirus by Multiplex PCR. The main presenting symptoms and signs in children with adenovirus detected by PCR were cough, fever, wheezing, and croups (90.9%, 81.1%, 63.6%, and 63.6%, respectively). The diagnosis in children with adenovirus was pneumonia in 72.7% and bronchitis in 27.7%. There were statistically insignificant differences in demographic, clinical, and hematological parameters between children with adenovirus and children negative to adenovirus by PCR.

Conclusion:

The clinical characteristics of respiratory infections with adenovirus vary upon the age of the patients and the immune status. Therefore, there is a requirement for an extensive study of adenovirus in respiratory infections in children with different ages and immune status.

Keywords: Children, Respiratory infection, Pneumonia, Adenovirus genotype 7, Adenovirus genotype 3, Multiplex PCR.