Distribution of Class 1 and 2 Integrons Among Salmonella Enterica Serovars Isolated from Iranian Patients

The Open Microbiology Journal 28 Feb 2019 RESEARCH ARTICLE DOI: 10.2174/1874285801913010063



Salmonella enterica has become increasingly resistant to antimicrobial agents, partly as a result of genes carried by integrons.


The aim of the present study was to investigate the prevalence of class 1 and 2 integrons and resistance to antimicrobial agents in clinical isolates of S. enterica.


This study included all Salmonella isolates, recovered from patients with salmonellosis, admitted to Medical Children Hospital, Tehran, Iran during 2015-2016. Bacterial isolates were identified using standard biochemical and agglutination tests. Antimicrobial susceptibility testing was performed according to the Clinical and Laboratory Standards Institute guidelines. The presence of class 1 and 2 integrons was investigated by Polymerase Chain Reaction (PCR) assay, using specific primers.


A total of 138 Salmonella strains were isolated and included in this study. Integrons were detected in 45 (32%) isolates. Class 1 and 2 integrons were detected in 24 (17.3%) and 21 (15.2%) isolates, respectively. All integron-positive isolates showed multidrug-resistant phenotypes. Resistance to more than three antimicrobial agents was observed in integron-positive isolates.


Our findings showed that integrons were widely distributed among S. enterica isolates in Tehran. Class 1 integrons are more prevalent than class 2 integrons in Salmonella isolates, and there is an association with MDR patterns. Therefore, these integrons are more likely to be involved in the distribution of resistant phenotypes in Salmonella strains.

Keywords: Antibiotic resistance, Integrons, Multidrug-Resistant (MDR), Salmonella enterica, Antimicrobial agents, Phenotypes.
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