RESEARCH ARTICLE
Evaluation of Biofilm Formation and Anti-biofilm Properties of Peganum Harmala and Crocus Sativus in Shigella Flexneri Clinical Isolates
Mahsa Jalili1, Mansour Amraei1, Nourkhoda Sadeghifard1, 2, Sobhan Ghafourian1, 2, *
Article Information
Identifiers and Pagination:
Year: 2019Volume: 13
First Page: 297
Last Page: 300
Publisher ID: TOMICROJ-13-297
DOI: 10.2174/1874285801913010297
Article History:
Received Date: 01/09/2019Revision Received Date: 18/11/2019
Acceptance Date: 20/11/2019
Electronic publication date: 31/12/2019
Collection year: 2019
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background:
Biofilm formation causes many serious problems in the treatment of bacterial infections. In addition, chronic infections due to biofilm formation can pose a huge burden to the health care systems. Also, many bacteria are biofilm producers as an important strategy for pathogenicity. Furthermore, the traditional use of herbal medicines such as Peganum harmala and Crocus sativus in Iran is interesting.
Objective:
The purpose of the current study was to investigate the biofilm formation in Shigella flexneri clinical isolates and to evaluate the anti-biofilm properties of P. harmala and C. sativus on Shigella flexneri clinical isolates.
Methods:
For the study purpose, Thirty S.flexneri clinical isolates were collected from Ahvaz, Iran. Then, the collected bacteria were subjected to biofilm formation assay. Afterward, P. harmala and C. sativus were applied as an anti-biofilm formation in S. flexneri.
Results & Conclusion:
Our results demonstrated that a significant number of samples were identified as strong biofilm producers. Then, P. harmala and C . sativus in a concentration of 30μg/ml and 60μg/ml were able to eradicate a strong biofilm formation in S. flexneri, respectively. In addition, it seems that more extensive studies and in vivo research should be done to confirm their properties.