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Arbuscular Mycorrhizal Fungi Increase the Phenolic Compounds Concentration in the Bark of the Stem of Libidibia Ferrea in Field Conditions



Emanuela Lima dos Santos1, 2, 3, *, Francineyde Alves da Silva2, Fábio Sérgio Barbosa da Silva1, 2, 3
1 Post-graduation in Cellular and Applied Molecular Biology, Institute of Biological Sciences, University of Pernambuco, 310, Arnóbio Marques Street, Santo Amaro – 50100130 - Recife, Brazil
2 University of Pernambuco, campus Petrolina, Laboratory of Mycorrhizal Technology (LTM/UPE)- Petrolina Center, BR 203, Km 2, 56328-900-Petrolina, Brazil
3 University of Pernambuco, campus Santo Amaro, Laboratory of Mycorrhizal Technology (LTM/UPE) – Recife Center, 310, Arnóbio Marques Street, Santo Amaro – 50100130 - Recife, Brazil


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© 2017 Santos et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Post-graduation in Cellular and Applied Molecular Biology, Institute of Biological Sciences, University of Pernambuco, 310, Arnóbio Marques Street, Santo Amaro – 50100130 - Recife, PE; University of Pernambuco, campus Petrolina, Laboratory of Mycorrhizal Technology (LTM/UPE)- Petrolina Center, BR 203, Km 2, 56328-900-Petrolina, PE; University of Pernambuco, campus Santo Amaro, Laboratory of Mycorrhizal Technology (LTM/UPE) – Recife Center, 310, Arnóbio Marques Street, Santo Amaro – 50100130 - Recife, PE- Brazil; Tel: 55+813183-3316; E-mails: emanuela_lima07@hotmail.com, emanuela_lima@oi.com.br


Abstract

Background:

Libidibia ferrea is a species particular to the caatinga presenting medicinal properties for containing bioactive compounds. The use of Arbuscular Mycorrhizal Fungi (AMF) can increase the production of biomolecules in the legume leaves; however, no light has been shed on the role of symbiosis in maximizing metabolites production in the bark of L. ferrea stem.

Objective:

The aim was to select AMF that are efficient at increasing the production of phenolic compounds with medicinal properties in the bark of the L. ferrea stem.

Methods:

The experiment was designed in randomized blocks with four inoculation treatments (plants pre-inoculated with Claroideoglomus etunicatum, with Gigaspora albida, with Acaulospora longula, and non-inoculated plants – control) with six repetitions. Thirteen months after the transplanting, the plants were pruned and the bark of the stem was collected; subsequently, this plant material was dried in a chamber. After the drying process, fractions of the bark of the stem were macerated in methanol. The extracts were further used for analyses of the biomolecules.

Results:

The flavonoids concentration had an increase of, respectively, 236% and 186% in relation to the control for the treatments with A. longula and C. etunicatum; plants inoculated with A. longula had an increase of 47% in total tannins concentration compared with the non-inoculated control – a benefit that the proanthocyanidins did not present.

Conclusion:

Applying inoculation with A. longula may be an alternative to increase the production of biomolecules of the secondary metabolism in the bark of the L. ferrea stem in field conditions.

Keywords: Caatinga, AMF, Bioactive compounds, Glomeromycota, Secondary metabolism, Field conditions.