RESEARCH ARTICLE


Isolation, Detection, and Characterization of Enterotoxigenic Bacteroides fragilis in Clinical Samples



Payam Fathi, Shaoguang Wu*
Department of Medicine, Division of Infectious Disease, Johns Hopkins University, Baltimore, MD, USA


Article Metrics

CrossRef Citations:
0
Total Statistics:

Full-Text HTML Views: 984
Abstract HTML Views: 1006
PDF Downloads: 258
ePub Downloads: 185
Total Views/Downloads: 2433
Unique Statistics:

Full-Text HTML Views: 591
Abstract HTML Views: 530
PDF Downloads: 151
ePub Downloads: 127
Total Views/Downloads: 1399



© Fathi and Wu; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Medicine, Division of Infectious Disease, Johns Hopkins University School of Medicine, Baltimore, MD, 21231, USA; E-mail: sgwu@jhmi.edu


Abstract

Bacteroides fragilis is an extensively studied anaerobic bacterium comprising the normal flora of the human gut. B. fragilis is known to be one of the most commonly isolated species from clinical samples and has been shown to cause a wide range of pathologies in humans [1, 2]. As an opportunistic pathogen B. fragilis can cause abscess formation and bacteremia [2]. Additionally in its enterotoxigenic form, B. fragilis is a known cause of diarrheal illness, is associated with inflammatory bowel disease, and has been recently characterized in patients with colon cancer [3-5]. As research in the field of the gut microbiome continues to expand at an ever increasing rate due to advances in the availability of next generation sequencing and analysis tools it is important to outline various molecular methods that can be employed in quickly detecting and isolating relevant strains of B. fragilis. This review outlines methods that are routinely employed in the isolation and detection of B. fragilis, with an emphasis on characterizing enterotoxigenic B. fragilis (ETBF) strains.

Keywords: Bacteroides fragilis, diarrheal illness, enterotoxigenicm form, human gut, non-enterotoxigenic.