Study Prevalence of Verotoxigenic E.coli Isolated from Urinary Tract Infections (UTIs) in an Iranian Children Hospital



Masoumeh Navidinia1, Abdollah Karimi1, Mohammad Rahbar2, 3, *, Fatemeh Fallah1, Raheleh Radmanesh Ahsani1, Mohammad Ali Malekan1, Mana Hadipour Jahromi1, Zari GholineJad1
1 Pediatric Infection Research Center, Mofid Children Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Departments of Microbiology, Iranian Reference Health Laboratory Iran, Ministry of Health and Medical Education, Tehran, Iran
3 Antimicrobial Resistance Center, Tehran University of Medical Sciences, Tehran, Iran


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© Navidinia et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Microbiology, Research Center and Reference Health Laboratories of Iran Hafez Street, Zartoshteian Ave, Keikhosrow Shahrookh alley , No 48,. Tehran, Iran; Tel: +98 2166728112-13; Fax: +9821 66728121; E-mail: rahbar_reflab@yahoo.com


Abstract

Background and Objectives:

Urinary tract infections (UTI) caused by enterohemorrhagic Escherichia coli (EHEC) is one of the most important diseases in infants and children. If there would not be any useful diagnosis and treatment it may be resulted in diseases such as acute renal failure, thrombocytopenia and hemolytic anemia. The aim of this study was to determine frequency of verotoxigenic E.coli isolates in urine of children with (UTIs) in Mofid children Hospital.

Methods:

During one year from September 2008 to august 2009, urine specimens were taken from children who suspected to UTI admitted to Mofid Children Hospital. E.coli strains that indicated beta hemolytic on sheep blood agar, negative sorbitol fermentation on SMAC (sorbitol macconky agar) and negative motility on SIM were tested by PCR and serologic (VITEC-RPLA kit) methods for detecting toxin genes and production of toxin, respectively.

Results:

Among 12572 urine specimens were taken from children admitted to Mofid hospital, we isolated 378 E.coli from urine samples which only 9 isolates were EHEC. Only five EHEC strains (55%) which produced vtx genes, were detected by serologic and PCR methods.

Conclusion:

The prevalence of urinary infections caused by EHEC strains is very significant because it causes aggravating pathologic effects. Thus we suggest rapid method for identification of this bacteria and proper treatment to Inhibition of unwanted complications.

Keywords: UTI, Enterohemorrhagic Escherichia coli( EHEC).