RESEARCH ARTICLE


Usefulness of the Paralens™ Fluorescent Microscope Adaptor for the Identification of Mycobacteria in Both Field and Laboratory Settings



Walter Kuhn1, *, Derek Armstrong2, Suzanne Atteberry1, Euline Dewbrey3, Diane Smith3, Nancy Hooper3
1 Department of Emergency Medicine, Center for Operational Medicine, Medical College of Georgia, 1120 15th Street, Augusta, Georgia 30912
2 MTW/SIM HIV/AIDS Care and Treatment Project, Addis Ababa, Ethiopia
3 Maryland State Department of Health and Mental Hygiene, Mycobacteriology Laboratory Section, 201 W. Preston Street, Baltimore, Maryland 21201


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© Kuhn et al.; Licensee Bentham Open.

open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

* Address correspondence to this author at the Department of Emergency Medicine, Center for Operational Medicine, Medical College of Georgia, 1120 15th Street, Augusta, Georgia, 30912, USA; Tel: (706) 721-4412; Fax: (706) 721-7718; E-mail- Tkuhn@mail.mcg.edu


Abstract

The presence of acid-fast bacilli (AFB) in laboratories has traditionally been demonstrated using the fluorochrome method, which requires a fluorescent microscope or the Ziehl-Neelsen (ZN) method employing light microscopy. Low sensitivity of the ZN method and high costs of fluoroscopy make the need for a more effective means of diagnosis a top priority, especially in developing countries where the burden of tuberculosis is high. The QBC ParaLens™ attachment (QBC Diagnostic Inc., Port Matilda, PA) is a substitute for conventional fluoroscopy in the identification of AFB. To evaluate the efficacy of the ParaLens LED (light-emitting diode) system, the authors performed a two-part study, looking at usefulness, functionality and durability in urban/rural health clinics around the world, as well as in a controlled state public health laboratory setting. In the field, the ParaLens was durable and functioned well with various power sources and lighting conditions. Results from the state laboratory indicated agreement between standard fluorescent microscopy and fluorescent microscopy using the ParaLens. This adaptor is a welcome addition to laboratories in resource-limited settings as a useful alternative to conventional fluoroscopy for detection of mycobacterial species.

Keywords: Tuberculosis, mycobacteria, fluorescent microscopy, acid-fast staining, paraLens.