A New Method for Isolating Host-Independent Variants of Bdellovibrio bacteriovorus Using E. coli Auxotrophs
Aliza Dashiff, Daniel E Kadouri*
Identifiers and Pagination:Year: 2009
First Page: 87
Last Page: 91
Publisher Id: TOMICROJ-3-87
Article History:Received Date: 5/5/2009
Revision Received Date: 13/5/2009
Acceptance Date: 15/5/2009
Electronic publication date: 2/6/2009
Collection year: 2009
open-access license: This is an open access article licensed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
Bdellovibrios are Gram-negative bacteria that are characterized by predatory behavior. Although Bdellovibrios exhibit an obligatory parasitic life cycle, it is possible to isolate Bdellovibrio variants that no longer require host cells for their growth. In this study, a new method for isolating Bdellovibrio bacteriovorus host-independent (HI) variants was developed. Filtered B. bacteriovorus prey cells were cultured with E. coli diaminopimelic acid (DAP) auxotrophs as host cells. Thereafter, the lysate was plated on DAP minus media, allowing only HI colonies to develop. Using this method, we have isolated numerous HI variants and demonstrated that the emergence of HI variants may be occurring at a higher frequency than was previously suggested.