RESEARCH ARTICLE
Influence of Nitrogen Source on 2,4-diacetylphloroglucinol Production by the Biocontrol Strain Pf-5
Hultberg M*, Alsanius B
Article Information
Identifiers and Pagination:
Year: 2008Volume: 2
First Page: 74
Last Page: 78
Publisher ID: TOMICROJ-2-74
DOI: 10.2174/1874285800802010074
Article History:
Received Date: 25/4/2008Revision Received Date: 12/5/2008
Acceptance Date: 16/5/2008
Electronic publication date: 30/5/2008
Collection year: 2008

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
The production of 2,4-diacetylphloroglucinol (DAPG) by the biocontrol agent Pseudomonas fluorescens Pf-5 was studied in nutrient-solution based media with varying nitrogen content. No production of DAPG was observed when organic nitrogen was omitted from the media, regardless of the inorganic nitrogen source used. Furthermore, a micromolar concentration range of organic nitrogen was insufficient to sustain production. When a millimolar concentration range of organic nitrogen was used, DAPG production was observed in the medium with ammonium as the inorganic nitrogen source. No production was observed in the treatments with ammonium and nitrate or nitrate only, despite growth of the bacterial strain being the same for all treatments. These results suggest that it is possible to manipulate the nutrient solution to increase the reliability and efficacy of biological control agents.