RESEARCH ARTICLE


Bacterial Concentration and Diversity within Repetitive Aliquots Collected from Replicate Continuous-Flow Bioreactor Cultures



Tawni L Crippen*, 1, Cynthia L Sheffield1, Kathleen Andrews1, Roy Bongaerts2, David J Nisbet1
1 Southern Plains Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, College Station, Texas, USA
2 Flow Cytometry Laboratory, Institute of Food Research, Norwich Research Park, Colney, Norwich, UK


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©Crippen et al.; Licensee Bentham Open

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.

* Address correspondence to this author at the Southern Plains Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, 2881 F & B Rd, College Station, Texas 77845, USA; Tel: (979) 260-3773; Fax: (979) 260 -9332; E-mail: crippen@ffsru.tamu.edu


Abstract

The aim of this study was to determine the reproducibility of small volume repeat sampling from replicate bioreactors with stabilized continuous-flow chicken cecal bacterial communities. Bacterial concentration and diversity were analyzed by phenotypic, biochemical and ribotype analysis. Significant differences in concentrations and variations in diversity were found in replicate bioreactors.

Keywords: Bioreactor, cecal, chicken, continuous-flow culture, repetitive sampling.