Bacterial Concentration and Diversity within Repetitive Aliquots Collected from Replicate Continuous-Flow Bioreactor Cultures
Tawni L Crippen*, 1, Cynthia L Sheffield1, Kathleen Andrews1, Roy Bongaerts2, David J Nisbet1
Identifiers and Pagination:Year: 2008
First Page: 60
Last Page: 65
Publisher Id: TOMICROJ-2-60
Article History:Received Date: 20/4/2008
Revision Received Date: 28/4/2008
Acceptance Date: 2/5/2008
Electronic publication date: 23/5/2008
Collection year: 2008
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.
The aim of this study was to determine the reproducibility of small volume repeat sampling from replicate bioreactors with stabilized continuous-flow chicken cecal bacterial communities. Bacterial concentration and diversity were analyzed by phenotypic, biochemical and ribotype analysis. Significant differences in concentrations and variations in diversity were found in replicate bioreactors.