Cloning, Purification, and Partial Characterization of the Halobacterium sp. NRC-1 Minichromosome Maintenance (MCM) Helicase
Nozomi Sakakibara*, 1, Mimi Han1, Claire R Rollor1, Rebecca C Gilson1, Courtney Busch2, Gunyoung Heo1, Zvi Kelman1
Identifiers and Pagination:Year: 2008
First Page: 13
Last Page: 17
Publisher Id: TOMICROJ-2-13
Article History:Received Date: 29/11/2007
Revision Received Date: 12/2/2008
Acceptance Date: 12/2/2008
Electronic publication date: 12/3/2008
Collection year: 2008
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.5/), which permits unrestrictive use, distribution, and reproduction in any medium, provided the original work is properly cited.
The MCM gene from the archaeon Halobacterium, with and without its intein, was cloned into an Escherichia coli expression vector, overexpressed and the protein was purified and antibodies were generated. The antibodies were used to demonstrate that in vivo only the processed enzyme, without the intein, could be detected.