RESEARCH ARTICLE


Large-Scale Evaluation of ureC (glmM) and SSA Conventional PCR for Rapid Direct Detection of Helicobacter pylori in Gastric Biopsies as Compared to rpoB-based Quantitative Real-Time PCR



Mohammed Akeel1, *, Atef Shehata2, 3, Ahmed Elhafey4, 5, Erwa Elmakki6, Thanaa Aboshouk7, Hussein Ageely6, Mohammed Salih Mahfouz8
1 Department of Anatomy, Faculty of Medicine, Jazan University, Jazan, Kingdom of Saudi Arabia
2 Department of Microbiology and Immunology, Faculty of Medicine, Jazan University, Jazan, Kingdom of Saudi Arabia
3 Department of Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt
4 Department of Pathology, Faculty of Medicine, Jazan University, Jazan, Kingdom of Saudi Arabia
5 Department of Pathology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt
6 Department of Internal Medicine, Faculty of Medicine, Jazan University, Jazan, Kingdom of Saudi Arabia
7 Department of Biochemistry, Faculty of Medicine, Jazan University, Jazan, Kingdom of Saudi Arabia
8 Department of Family and Community Medicine, Faculty of Medicine, Jazan University, Jazan, Kingdom of Saudi Arabia


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Creative Commons License
© 2022 Akeel et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the Department of Anatomy, Faculty of Medicine, Jazan University, Jazan, Kingdom of Saudi Arabia; Tel: 00966561120210; E-mail: m.akeel@jazanu.edu.sa


Abstract

Background:

Conventional polymerase chain reaction (PCR)-based methods play a major role in the direct detection of H. pylori in clinical specimens, with time-saving as compared to culture-based methods. However, specificity and sensitivity vary among different varieties of these PCRs, which consequently could affect the accuracy of diagnosis of H. pylori infection. The study aimed to evaluate the utility of ureC (glmM) and SSA conventional PCR methods for rapid direct detection of H. pylori by comparing them with rpoB-based quantitative real-time PCR.

Methods:

A total of 402 non-repeated gastric biopsy specimens were subjected to DNA extraction followed by conventional ureC (glmM) and SSA PCR, and rpoB-based quantitative real-time PCR, which was used as the gold standard.

Results:

H. pylori was detected in 119 (29.6%), 126 (31.34%), and 187 (46.5%) of the tested specimens using ureC (glmM) PCR, SSA PCR, and real-time quantitative PCR, respectively. The specificity of the SSA PCR was higher than that of ureC (glmM) PCR (99.5% and 98.6%, respectively). The SSA PCR was more sensitive than the ureC (glmM), (66.8% and 62%, respectively). The diagnostic accuracy of SSA PCR (84.33%) was higher than that of ureC (glmM) PCR (81.59%).

Conclusion:

Overall, SSA PCR is more specific, sensitive, and diagnostically accurate than ureC (glmM) PCR, giving the SSA PCR assay superiority as a simple, rapid, and accurate diagnostic tool for direct detection of H. pylori in gastric tissue specimens.

Keywords: Helicobacter pylori, ureC (glmM) PCR, SSA PCR, Real-time PCR, Diagnostic accuracy, rpoB-based quantitative real-time, Gastric tissue.