RESEARCH ARTICLE


Molecular Study of Escherichia albertii in Pediatric Urinary Tract Infections



Maysaa El Sayed Zaki1, *, Abd ElRahman Eid2, Samah Sabry El-Kazzaz3, Amr Mohamed El-Sabbagh3
1 Department of Clinical Pathology, Faculty of Medicine, Mansoura University, Elgomhoria street, Mansoura, 50, 35516, Egypt
2 Department of Pediatric, Faculty of Medicine, Mansoura University, Elgomhoria street, Mansoura, 50, 35516, Egypt
3 Department of Medical Microbiology and Immunology, Faculty of Medicine, Mansoura University, Elgomhoria Street, Mansoura, 50, 35516, Egypt


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Creative Commons License
© 2021 Zaki et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to these authors at the Department of Clinical Pathology, Professor, Faculty of Medicine, Mansoura University, Elgomhoria street, Mansoura, 50, 35516, Egypt; E-mail: maysaazaki5@hotmail.com


Abstract

Background:

There are insufficient data about the presence of E. albertii as a causative organism in urinary tract infection in pediatric patients. Objective: The present study aimed to detect E. albertii by polymerase chain reaction (PCR) for detection of uidA, mdh, and lysP genes among isolated E.coli from children with urinary tract infection.

Methods:

The present study was a cross-sectional retrograde study which was carried out on 100 isolates of phenotypically confirmed E.coli detected in urine samples of children suffering from urinary tract infection. The isolates were subjected to molecular identification by PCR for uidA, mdh, and lysP genes.

Results:

E. albertii was identified by PCR in 7% of the isolates and E.coli was identified in 93% of the isolates. Two mdh and lysP genes were detected for E. albertii and the uidA gene for E. coli. E. albertii isolates had marked resistance to gentamicin (71.4%), followed by resistance to ciprofloxacin (57.1%), meropenem and imipenem (42.9% each) and ESBL activity by double discs method was reported in 57.1% of the isolates. However, none of the isolates had shown resistance to nalidixic acid and only one isolate had resistance to norfloxacin. There was a statistically insignificant difference between resistance to the used antibiotics such as aztreonam (P=0.083), ampicillin/clavulanate (P=0.5), ciprofloxacin (P=0.69), gentamicin (P=0.3) and ceftazidime (P=1.00).

Conclusion:

The present study highlights the emergence of E. albertii as a pathogen associated with urinary tract infections in children. There is marked antibiotic resistance of this pathogen, especially toward extended spectrum beta-lactams antibiotics. The identification method depends mainly on genetic studies. Further longitudinal studies with large number of patients are required to verify the accurate prevalence of this bacterium.

Keywords: E. albertii, Children, PCR, uidA, mdh, lysP genes.