Plant Growth-Promoting and Bio-Control Activity of Micrococcus luteus Strain AKAD 3-5 Isolated from the Soybean (Glycine max (L.) Merr.) Rhizosphere

Anamika Dubey1, Ashwani Kumar1, *, Mohammed Latif Khan2, Devendra Kumar Payasi3
1 Metagenomics and Secretomics Research Laboratory, Department of Botany, Dr. Harisingh Gour University (A Central University), Sagar 470003, Madhya Pradesh, India.
2 Department of Botany, Dr. Harisingh Gour University (A Central University), Sagar470003, Madhya Pradesh, India.
3 Jawaharlal Nehru Krishi Vishwa Vidyalaya, Regional Agriculture Research Station, Sagar, MP470002, India.



Applications of bio inoculants for improving crop productivity may be an eco-friendly alternative to chemical fertilizers. Rhizosphere or soil-inhabiting beneficial microbes can enhance plant growth and productivity through direct and indirect mechanisms, i.e., phosphate solubilization, nutrient acquisition, phytohormone production, etc.


This study is based on the hypothesis that diseases resistant plants can act as a source of potential microbes that can have good plant growth-promoting traits and bio-control potential.


In this study, we have isolated the rhizobacterial strains (AKAD 2-1, AKAD 2-10, AKAD 3-5, AKAD 3-9) from the rhizosphere of a disease-resistant variety of soybean (JS-20-34) (Glycine max L). These bacterial strains were further screened for various plant growth-promoting traits (phosphate solubilization, indole acetic acid (IAA), ammonia, biofilm, HCN, Exopolysaccharide (EPS), and enzyme production activity (catalase, cellulase, and chitinase)).


Among four, only AKAD 3-5 have shown plant-growth-promoting and biocontrol (98%) activity against Fusarium oxysporum. Morphological, biochemical, and molecular characterization (16S rRNA) revealed that this rhizobacterial isolate AKAD 3-5 closely resembles Micrococcus luteus (Gene bank accession: MH304279).


Here, we conclude that this strain can be utilized to promote soybean growth and other crops grown under varied soil stress conditions.

Keywords: Rhizobacteria, PGPR, Rhizosphere, Phosphate solubilization, Soybean.

Abstract Information

Identifiers and Pagination:

Year: 2021
Volume: 15
Publisher Item Identifier: EA-TOMICROJ-2021-HT2-1594-4

Article History:

Electronic publication date: 15/7/2021
Collection year: 2021

© Dubey et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at Metagenomics & Secretomics Research laboratory, Department of Botany, Dr. Harisingh Gour University (A Central University), Sagar (M.P.) India; Tel: 91-7697432012; E-mail:; 0000-0002-8453-3183