Plant Growth-Promoting and Bio-Control Activity of Micrococcus luteus Strain AKAD 3-5 Isolated from the Soybean (Glycine max (L.) Merr.) Rhizosphere



Anamika Dubey1, Ashwani Kumar1, *, Mohammed Latif Khan2, Devendra Kumar Payasi3
1 Metagenomics and Secretomics Research Laboratory, Department of Botany, Dr. Harisingh Gour University (A Central University), Sagar 470003, Madhya Pradesh, India.
2 Department of Botany, Dr. Harisingh Gour University (A Central University), Sagar470003, Madhya Pradesh, India.
3 Jawaharlal Nehru Krishi Vishwa Vidyalaya, Regional Agriculture Research Station, Sagar, MP470002, India.

Abstract

Background:

Applications of bio inoculants for improving crop productivity may be an eco-friendly alternative to chemical fertilizers. Rhizosphere or soil-inhabiting beneficial microbes can enhance plant growth and productivity through direct and indirect mechanisms, i.e., phosphate solubilization, nutrient acquisition, phytohormone production, etc.

Objective:

This study is based on the hypothesis that diseases resistant plants can act as a source of potential microbes that can have good plant growth-promoting traits and bio-control potential.

Methods:

In this study, we have isolated the rhizobacterial strains (AKAD 2-1, AKAD 2-10, AKAD 3-5, AKAD 3-9) from the rhizosphere of a disease-resistant variety of soybean (JS-20-34) (Glycine max L). These bacterial strains were further screened for various plant growth-promoting traits (phosphate solubilization, indole acetic acid (IAA), ammonia, biofilm, HCN, Exopolysaccharide (EPS), and enzyme production activity (catalase, cellulase, and chitinase)).

Results:

Among four, only AKAD 3-5 have shown plant-growth-promoting and biocontrol (98%) activity against Fusarium oxysporum. Morphological, biochemical, and molecular characterization (16S rRNA) revealed that this rhizobacterial isolate AKAD 3-5 closely resembles Micrococcus luteus (Gene bank accession: MH304279).

Conclusion:

Here, we conclude that this strain can be utilized to promote soybean growth and other crops grown under varied soil stress conditions.

Keywords: Rhizobacteria, PGPR, Rhizosphere, Phosphate solubilization, Soybean.


Abstract Information


Identifiers and Pagination:

Year: 2021
Volume: 15
Publisher Item Identifier: EA-TOMICROJ-2021-HT2-1594-4

Article History:

Electronic publication date: 15/7/2021
Collection year: 2021

© Dubey et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


* Address correspondence to this author at Metagenomics & Secretomics Research laboratory, Department of Botany, Dr. Harisingh Gour University (A Central University), Sagar (M.P.) India; Tel: 91-7697432012; E-mail: ashwaniiitd@hotmail.com; 0000-0002-8453-3183