Molecular Study of Class1 and 2 Integrons in Methicillin-resistant Staphylococcus Aureus



Maysaa El Sayed Zaki1, *, Osama Ahmed Faried2, Karim Montaseer3
1 Clinical Pathology Department, Mansoura Faculty of Medicine, Mansoura University, Mansoura, Egypt
2 Medical Microbiology and Immunology Department, Faculty of Medicine, Beni-Suef University, Beni Suef, Egypt
3 Clinical Pathology Department-Helwan Faculty of Medicine, Helwan University, Cairo, Egypt

Abstract

Background and Aim:

Methicillin-Resistant Staphylococcus aureus (MRSA) had appeared in the last few decades and became a significant health problem. The study aimed to evaluate the prevalence of class 1 and class 2 integrons among MRSA clinical isolates from different healthcare-associated infections (HAIs) and the prevalence of multi-drug resistance (MDR) in these isolates.

Materials and Methods:

Two hundred Staphylococcus aureus (S. aureus) isolates from different HAIs were evaluated for the presence of the mecA gene, class 1 (intI1) and class 2 (intI2) integrons via the polymerase chain reaction (PCR) beside the determination of antibiotics sensitivity.

Results:

MRSA was determined by resistance to cefoxitin in 152 isolates (76%) of isolated S. aureus. The molecular study showed the prevalence of mecA, intI1, and intI2 genes among the isolated MRSA strains as follows: 100%, 70.4%, 13.1, respectively, and there were 12 isolates (7.9%) that had both intI1 and intI2 genes. There was a significant high incidence of resistance to gentamicin (P=0.01), amikacin (P=0.005), tobramycin (P=0.035), erythromycin (P=0.035), clindamycin (P=0.08), levofloxacin (P=0.0001) and ciprofloxacin (P=0.0001) in MRSA strains positive for intI1 and/or intI2 genes compared to MRSA strains negative for intI genes and non-methicillin resistant S. aureus (Non- MRSA) strains. There was a significantly high prevalence of MDR among MRSA strains positive for intI1 and/or intI2 genes (62.8%) compared to MRSA strains negative for intI genes (28.8%) and Non- MRSA strains (8.3%), (P=0.0001).

Conclusion:

The present study revealed a high prevalence of MRSA strains among S. aureus clinical isolates with a high prevalence of the mecA gene in those isolates. There was also a high prevalence of intI1gene but not intI2 gene. The mecA and intI genes were associated with the development of MDR in S. aureus.

Keywords: Methicillin-Resistant Staphylococcus aureus, Class 1 integron, Class 2 integron multidrug resistance, Mobile genetic elements, SCCmec, Cefoxitin.


Abstract Information


Identifiers and Pagination:

Year: 2021
Volume: 15
Publisher Item Identifier: EA-TOMICROJ-2020-65

Article History:

Electronic publication date: 11/1/2021
Collection year: 2021

© 2021 Zaki et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


* Address correspondence to this author at Department of Clinical Pathology, Mansoura Faculty of Medicine, Egypt, Egypt-Mansoura-Elgomhoria street-31554, Egypt; Tel: 00220502258877; E-mail: may_s65@hotmail.com