RESEARCH ARTICLE

False-Positive Diagnostics of Bordetella Pertussis using IS481 PCR is Limited in Danish Patients

The Open Microbiology Journal 28 Feb 2019 RESEARCH ARTICLE DOI: 10.2174/1874285801913010051

Abstract

Background:

Bordetella pertussis is routinely detected using real-time PCR based on the multicopy insertion sequence IS481, which is not specific for Bordetella pertussis.

Objective:

The aim of this retrospective study was to evaluate the proportion of other Bordetella species misidentified as Bordetella pertussis using IS481-targeted real-time PCR.

Methods:

Clinical specimens from 228 Danish patients (median age 15 years, 0 to 90 years old) formerly identified as positive for Bordetella pertussis (IS481+) by routine PCR in 2011-2015, were subjected to real-time PCR targeting the insertion sequences IS1002 and IS1001.

Results:

The results showed that 2.3% of the samples were false-positive for Bordetella pertussis.

Conclusion:

In conclusion, we found that misidentification of Bordetella pertussis using IS481 PCR is limited in Danish patients.

Keywords: Bordetella pertussis, Real-time PCR, IS481, IS1002, IS1001, False-positive.
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