RESEARCH ARTICLE


Antibiotic Resistance Pattern and Frequency of PER-1, SHV-1 and AMPC Type B-Lactamase Genes in Pseudomonas aeruginosa Isolated from Clinical Samples



Fatemeh F. Amoudizaj1, *
iD
, Elnaz Aghayi2, Milad G. Matin3, Nayemeh Soltani3, Pejman Mala3
1 Department of Genetic, Tabriz Branch, Islamic Azad University, Tabriz, Iran
2 Department of Molecular Biology, Bonab Branch, Islamic Azad University, Bonab, Iran
3 Department of Microbiology, Urmia Branch, Islamic Azad University, Urmia, Iran


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Creative Commons License
© 2019 Amoodizaj et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at Department of Genetic, Tabriz Branch, Islamic Azad University, Tabriz, Iran; E-mail: fatemeh.firoozi96@ yahoo.com; Tel: +98-9395572387


Abstract

Background:

The existence of Extended Spectrum B-lactamase (ESBL) genes plays an important role in spreading B-lactam antibiotic resistance in the producing strains of these enzymes. The resistance of gram-negative bacteria, such as Pseudomonas aeruginosa, to different antimicrobial agents, especially B-lactams, has increasingly been reported.

Objective:

This study was conducted to determine the prevalence of TEM-1and VEB-1 beta-lactamases gene in P. aeruginosa isolates through Polymerase Chain Reaction (PCR) method.

Methods:

100 clinical isolates of P. aeruginosa were collected from different clinical samples. The antibiotic susceptibility was examined by the disc diffusion method. The presence of PER-1, SHV-1 and AMPC genes was detected by PCR method.

Results:

Out of the studied P. aeruginosa isolates, 7, 9 and 37 isolates were positive for PER-1, SHV-1 and AMPC B-lactamases resistance genes, respectively. Patients with urinary infection had the most resistant isolates. All isolates (100%) were sensitive to polymyxin B.

Conclusion:

Antibiotic resistance in isolates of Pseudomonas can be caused by B-lactamases resistance genes. Noticing the increasing rate of the ESBLs producing strains, using the appropriate treatment protocol based on the antibiogram pattern of the strains is highly recommended.

Keywords: Pseudomonas aeruginosa, B-Lactamase, PER-1 gene, SHV-1 gene, AMPC gene, Antibiotic resistance, Polymerase chain reaction.