Bark of the Stem of Libidibia Ferrea Associated with Mycorrhizal Fungi: An Alternative to Produce High Levels of Phenolic Acids

Francineyde Alves da Silva1, *, Wliana Alves Viturino da Silva2, Magda Rhayanny Assunção Ferreira2, Luiz Alberto Lira Soares2, Fábio Sérgio Barbosa da Silva3, 4
1 Laboratório de Tecnologia Micorrízica – Universidade de Pernambuco – Campus Petrolina; Rodovia BR 203, Km 2, s/n - Vila Eduardo, Petrolina, 56328-903, PE-Brazil.
2 Laboratório de Farmacognosia, Departamento de Farmácia – CCS, Universidade Federal de Pernambuco, Av. Prof. Arthur Sá, S/N Cidade Universitária 50740521 – Recife, PE – Brazil.
3 Laboratório de Análises, Pesquisas e Estudos em Micorrizas, Universidade de Pernambuco; Rua Arnóbio Marques, 310, Santo Amaro – 50100130 - Recife, PE-Brazil.
4 Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada, Instituto de Ciências Biológicas, Laboratório de Análises, Pesquisas e Estudos em Micorrizas – LAPEM, ICB/Universidade de Pernambuco, Rua Arnóbio Marques, 310, Santo Amaro – 50100130, Recife, PE-Brazil.

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© 2018 Alves da Silva et al.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: ( This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Correspondence: Address correspondence to this author at the Laboratório de Tecnologia Micorrízica, Universidade de Pernambuco – Campus Petrolina; Rodovia BR 203, Km 2, s/n - Vila Eduardo, Petrolina, 56328-903, PE-Brazil; Tel: +55873866-6468; E-mail:



The use of microorganisms such as Arbuscular Mycorrhizal Fungi (AMF) may represent a sustainable biotechnological alternative for the cultivation of medicinal plants to facilitate plant growth, in addition to increasing the production of secondary compounds. These fungi are associated with Libidibia ferrea, a species which produces gallic and ellagic acid, compounds with preventive properties against cancer and diabetes complications.


The objective of this paper was to verify whether the stem bark of L. ferrea concentrates higher amounts of gallic and ellagic acids when inoculated with Claroideoglomus etunicatum, Gigaspora albida and Acaulospora longula.


The extractive methanolic solutions from the barks of L. ferrea were analyzed by RP-HPLC in order to establish the contents of gallic and ellagic acids.


The application of fungus Claroideoglumus etunicatum was more efficient at increasing the concentration of gallic acid (18%) and ellagic acid (45.2%) in the stem bark of L. ferrea in comparison to the control. In contrast, plants inoculated with Acaulospora longula benefited only with the increase in the amount of gallic acid if compared with the non-inoculated plants.


The mycorrhizal technology may be an alternative to the cultivation of L. ferrea with higher concentrations of both gallic and ellagic acids in the stem bark, providing a promissory strategy to produce high quality herbal materials for the production of herbal medicines.

Keywords: Phenolic content, Mycorrhizal association, Glomeromycotina, Libidibia ferrea, Gallic acid, Ellagic acid.