Identification of Bacteria in the Sputum of a Cystic Fibrosis patient; A Comparison of Phenotypic and Molecular Methods



Mubarak Alfaresi*, Bassam Mahboub
College of Medicine, University of Sharjah, UAE


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© 2017 Alfaresi and Mahboub.

open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

* Address correspondence to this author at the College of Medicine, University of Sharjah, Alain, PO BOX 1819, UAE; Tel: 00971504411755; E-mail: Mubarak.alfaresi@skgh.ae


Abstract

Background:

Cystic fibrosis (CF), caused by mutations in the CF transmembrane conductance regulator gene, is a common autosomal recessive disease. Accurate isolation and identification of the bacteria underlying these infections are is critical to the therapeutic management of CF.

Objective:

To compare phenotypic bacterial identification with a molecular method in a CF patient sputum.

Methods:

Bacterial identification done by standard microbiological method from a CF patient. Same sample underwent a molecular method involving 16S rDNA amplification, cloning, and sequencing.

Results:

All isolated bacteria from culture were also found after cloning PCR Product. Conversely, 9 pathogenic bacterial species were only detected after PCR and cloning.

Conclusion:

This study supports prior suggestions that a sequence-based molecular approach to clinical microbiology can significantly enhance the standard clinical culture-based view.