SHORT COMMUNICATION
Identification of Bacteria in the Sputum of a Cystic Fibrosis patient; A Comparison of Phenotypic and Molecular Methods
Mubarak Alfaresi*, Bassam Mahboub
Article Information
Identifiers and Pagination:
Year: 2017Volume: 11
First Page: 384
Last Page: 386
Publisher ID: TOMICROJ-11-384
DOI: 10.2174/1874285801711010384
Article History:
Received Date: 31/8/2017Revision Received Date: 8/12/2017
Acceptance Date: 10/12/2017
Electronic publication date: 29/12/2017
Collection year: 2017
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at: (https://creativecommons.org/licenses/by/4.0/legalcode). This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Background:
Cystic fibrosis (CF), caused by mutations in the CF transmembrane conductance regulator gene, is a common autosomal recessive disease. Accurate isolation and identification of the bacteria underlying these infections are is critical to the therapeutic management of CF.
Objective:
To compare phenotypic bacterial identification with a molecular method in a CF patient sputum.
Methods:
Bacterial identification done by standard microbiological method from a CF patient. Same sample underwent a molecular method involving 16S rDNA amplification, cloning, and sequencing.
Results:
All isolated bacteria from culture were also found after cloning PCR Product. Conversely, 9 pathogenic bacterial species were only detected after PCR and cloning.
Conclusion:
This study supports prior suggestions that a sequence-based molecular approach to clinical microbiology can significantly enhance the standard clinical culture-based view.
