In Silico Design of a Chimeric Protein Containing Antigenic Fragments of Helicobacter Pylori; A Bioinformatic Approach
Amir Ghasemi*Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran
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Electronic publication date: 16/11/2017
Collection year: 2017
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© 2017 Amir Ghasemi.
open-access license: This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International Public License (CC-BY 4.0), a copy of which is available at:
https://creativecommons.org/licenses/by/4.0/legalcode. This license permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
* Address correspondence to this author at Department of Microbiology and Immunology, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, Iran; Tel: 00989123595610; E-mail: ghasemia77@yahoo.com
REVISED VERSION NOVEMBER 2017
The following list provides a description of the changes made to the publication since the original version of the article entitled “In Silico Design of a Chimeric Protein Containing Antigenic Fragments of Helicobacter pylori; A Bioinformatic Approach” was published online in May 2016.
PAGE 97:
In the Abstract, the Following Text Appears:
“In order to obtain such benefit in H. pylori vaccine study, a chimeric gene containing four fragments of FliD sequence (1-600 bp), UreB (327-334 bp),VacA (744-805 bp) and CagL(51-100 bp) which have a high density of B- and T-cell epitopes was designed.”
This Should be:
“In order to obtain such benefit in H. pylori vaccine study, a chimeric gene containing four fragments of FliD sequence (1-600 bp), UreB (327-385 bp),VacA (744-805 bp) and CagL(51-100 bp) which have a high density of B- and T-cell epitopes was designed.”
PAGE 98:
In the paragraph 4, the following text appears:
“Urease B has been widely investigated as a potential antigen for the development of prophylactic and therapeutic vaccines against H. pylori infection [32, 33]. UreB(327-334) is considered as a good B cell epitope and has been found to be protective in mice [34, 35]”
This Should be:
“Urease B has been widely investigated as a potential antigen for the development of prophylactic and therapeutic vaccines against H. pylori infection [32, 33]. UreB (327-385) is considered as a good B cell epitope and has been found to be protective in mice [34, 35].”
PAGE 100:
In the Results, the Following Text Appears:
“The nominated sequences for designing of chimeric construct were FliD (1-600), UreB (327-334), VacA (744-805) and CagL (51-100).”
This Should be:
“The nominated sequences for designing of chimeric construct were FliD (1-600), UreB (327-385), VacA (744-805) and CagL (51-100).”
PAGE 102:
In the Paragraph 3, the Following Text Appears:
“The secondary structure of the chimeric protein was predicted by several online programs, and the best result was achieved by GOR-IV as shown in Fig. (4). Results indicated total residues of 785 which were made up 140 strands (17.83%), 299 helices (38.09%) and 346 random coils (44.08%). No predicted signal peptide was identified in the initial region of the protein sequence.”
This Should be:
“The secondary structure of the chimeric protein was predicted by several online programs, and the best result was achieved by GOR-IV as shown in Fig. (4). Results indicated total residues of 790 which were made up 140 strands (17.72%), 299 helices (37.85%) and 351 random coils (44.43%). No predicted signal peptide was identified in the initial region of the protein sequence.”
PAGE 107:
In the Discussion, the Following Text Appears:
Based on our finding, a chimeric protein including immunodominant epitopes from different antigenic proteins such as FliD (1-600), UreB (327-334), VacA (744-805) and CagL (51-100) would likely induce strong comprehensive protective immunity.
This Should be:
Based on our finding, a chimeric protein including immunodominant epitopes from different antigenic proteins such as FliD (1-600), UreB (327-385), VacA (744-805) and CagL (51-100) would likely induce strong comprehensive protective immunity.
PAGE 108:
In the Conclusion, the Following Text Appears:
“Our data showed that the possibility of successful production of a large chimeric protein composing of four domains FliD (1-600), UreB(327-334), VacA (744-805) and CagL (51-100) in the prokaryotic host.”
This Should be:
“Our data showed that the possibility of successful production of a large chimeric protein composing of four domains FliD (1-600), UreB(327-385), VacA (744-805) and CagL (51-100) in the prokaryotic host.”
