Isolation and Culturing Axenic Microalgae: Mini–Review
Saúl Fernandez-Valenzuela1, Francisca Chávez-Ruvalcaba2, Julio Cesar Beltran-Rocha3, Pilar Morales San Claudio1, Raúl Reyna-Martínez1, *
Microalgae have several applications in nutraceuticals, pharmaceuticals, cosmetics, biofuel production, and bioremediation, among other fields. Isolation and purification are extremely important for obtaining axenic cultures of microalgae from different environments and crucial for their biotechnological applications, but it is not an easy task. In view of the above, it is fundamental to know the classical and advanced techniques and examples of how scientists from around the globe have applied such methods to isolate several genera and the impact of each step on successful algal purification. This review provides a brief and simple explanation of the methodology for sampling, growth, obtention of unialgal, and posterior axenic culture, which will facilitate the development of novel microalgae-related discoveries and applications for new researchers.
* Address correspondence to this author at the Biotechnology Lab, Faculty of Chemical Sciences, Autonomous University of Nuevo León, San Nicolas de los Garza, 66455, México; E-mail: email@example.com